Donor Horse Serum


Donor Horse Serum is obtained from closed donor horse herds that receive regular veterinary inspection and care.  Donor Horse Serum will support the growth of a wide variety of mammalian cells, providing a cost-effective alternative for fetal bovine serum.

Download SDSProduct NameProduct Code
Donor Horse SerumHSS0200

Collection, Processing & Origin:

Donor Horse Serum used by Biologos meets all USDA requirements for animal products.  The Donor Horse Serum has low endotoxin and low hemoglobin values and the manufacturing process meets applicable requirements for animal products. The Donor Horse Serum is traceable back to the date and location of collection. In addition, all Donor Horse Serums used for processing must meet Biologos’ strict quality requirements for raw material.

Raw Materials:

Donor Horse blood is collected from prescreened donor animals that are maintained in controlled herds. The whole blood is processed to produce a Donor Horse Serum product that is frozen and shipped to Biologos for filtration and packaging.


Approved lots of raw Donor Horse Serum are thawed under controlled conditions and sterile filtered by a process that uses a series of filters in descending pore size, utilizing 0.2µm filters for the final filtration step. Filling takes place in a laminar flow hood certified to maintain ISO Class 5 conditions. The Donor Horse Serum is aseptically dispensed into gamma irradiated, sterile PETG or HDPE bottles and LDPE bioprocess bags (20-100 liter capacity). Filled containers are immediately labeled and frozen, and then maintained at temperatures less than -5°C to preserve the product quality.

Quality Control Testing

Microbiological Testing:

Each lot of Donor Horse Serum is tested to confirm the absence of bacterial or fungal contamination. Each lot of Donor Horse Serum is also tested to confirm the absence of mycoplasma contamination.

Virus Testing:

Donor Horse Serum is tested for adventitious agents using modified procedures adapted from the Code of Federal Regulations, Title 9, Section 113.53, “Requirements for Ingredients of Animal Origin”.

Storage and Handling:

Donor Horse Serum can be supplied in gamma irradiated, sterile PETG or HDPE bottles and LDPE bioprocess bags (20-100 liter capacity). We recommend that the Donor Horse Serum be stored frozen at a temperature of -5°C to -20°C. Multiple freeze-thaw cycles of the Donor Horse Serum should be avoided as this may lead to deterioration of the product. Always use aseptic techniques when handling the serum and aliquot into sterile containers.


Donor Horse Serum is shipped frozen by truck or next day air in insulated containers packed with dry ice.

What is Donor Horse Serum?

Donor Horse Serum is the clear, red-orange fluid that is obtained from separating blood, sourced from donor horse herds, in to its solid and liquid components.  Donor Horse Serum, like other animal sera, can be used as a supplement to basal growth medium in cell culture. Sera, like donor horse serum, is an important component of cell culture media. It serves as a source for amino acids, proteins, fat-soluble vitamins (such as A, D, E, and K), carbohydrates, lipids, hormones, growth factors, minerals, and additional trace elements.


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