Dulbecco’s Modified Eagle’s Medium (DMEM)

Product Overview


The product is intended to be used in vitro, in laboratory only. Do not use it in therapy, human or veterinary applications.

DMEM is a liquid serum-free media formulation and a variant of Basal Medium Eagle (BME). DMEM is modified to contain up to four times more vitamins and amino acids, and two to four times more glucose than EMEM. This modified composition makes DMEM suitable for more specific cell types; specifically, as a supplement in cell culture application.

Additional Information:

What is DMEM Media?
The Difference Between DMEM and EMEM
DMEM Media Composition
DMEM Storage and Handling
DMEM Uses

What is DMEM Media?

DMEM is a liquid serum-free media and a variant of BME, or Basal Medium Eagle, modified to contain up to four times more vitamins and amino acids, and two to four times more glucose than EMEM. DMEM’s composition makes it an ideal supplement for most cell culture media applications.

However, like EMEM (Essential Modified Eagle’s Medium), DMEM does not contain proteins or agents required to support growth and may need a serum or custom formulation for some cell culture media applications.

The Difference Between DMEM and EMEM?

DMEM and EMEM are liquid serum-free media formulations and variations of Basal Medium Eagle (BME). Both mediums lack protein and contain the amino acids, salts, glucose and vitamins, required to provide a cell with energy and maintain cells in tissue culture, but the DMEM formulation is modified to contain up to four times more vitamins and amino acids, and two to four times more glucose than EMEM.

This modified composition makes DMEM suitable for more specific cell types.

DMEM Media Composition

DMEM’s composition varies based on the chosen formulation for a specific application. The standard DMEM composition includes four times more vitamins and amino acids than the Basal Medium Eagle medium, from which it is derived. Custom DMEM composition varies and is available upon request from the reagent manufacturer, Biologos.

To request DMEM Composition, email: [email protected]

DMEM Storage and Handling

The DMEM Solution is supplied in gamma irradiated, sterile PETG or PETE bottles. We recommend that the DMEM Solution be stored refrigerated at a temperature of 2oC to 8oC. Always use aseptic techniques when handling the DMEM Solution.

When stored properly, the DMEM can have a shelf life of up to 2 years.

DMEM Uses

Dulbecco’s Modified Eagle’s Medium, DMEM, is a liquid media supplement used to maintain cells in culture.

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Available Products

LDH0011 - DMEM, Dulbecco’s Modified Eagle Medium (1X) – w/High Glucose, Sodium Pyruvate & Phenol Red; w/o L-Glutamine

LDH0010 - DMEM, Dulbecco’s Modified Eagle Medium (1X) – w/High Glucose & Sodium Pyruvate; w/o L-Glutamine & Phenol Red

LDH0001 - DMEM, Dulbecco’s Modified Eagle Medium (1X) – w/High Glucose & Phenol Red; w/o L-Glutamine & Sodium Pyruvate

LDH0000 - DMEM, Dulbecco’s Modified Eagle Medium (1X) – w/High Glucose; w/o L-Glutamine, Sodium Pyruvate & Phenol Red

LDH9111 - DMEM, Dulbecco’s Modified Eagle Medium (1X) – w/High Glucose, HEPES Buffer Sodium Pyruvate & Phenol Red; w/o L-Glutamine

LDH9101 - DMEM, Dulbecco’s Modified Eagle Medium (1X) – w/High Glucose, HEPES Buffer, L-Glutamine & Phenol Red; w/o Sodium Pyruvate

LDH9000 - DMEM, Dulbecco’s Modified Eagle Medium (1X) – w/High Glucose & HEPES Buffer; w/o L-Glutamine, Sodium Pyruvate & Phenol Red

LDFH0111 - Nut. F10 MIX/Dul. MEM 1:1 MIX

LDF0111 - Nutrient F12/Dulbecco’s MEM

LDLX111 - Dulbecco’s MEM(10X), Low Glucose

LDL0111 - Dulbecco’s MEM(1X) w/ 1000 mg Glucose

LDHA101 - Dulbecco’s MEM (1X) w/HEPES w/o NAHCO3

LDH9001 - Dulbecco’s MEM (1X) w/HEPES

LDH0100 - Dulbecco’s MEM (1X)

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