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Dulbecco’s Modified Eagle’s Medium (DMEM)

Advanced customization of your formulation from production to delivery.

Our experts provide you with premium formulations tailored for your specific application, including preferred delivery systems and quantity. Your formulations are stocked and stored in our temperature-controlled facility to ensure product quality, reliability and immediate availability.

The product is intended to be used in vitro, in laboratory only. Do not use it in therapy, human or veterinary applications.

DMEM is a liquid serum-free media and a variant of BME, or Basal Medium Eagle, modified to contain up to four times more vitamins and amino acids, and two to four times more glucose than EMEM; making it an ideal supplement for most cell culture media applications.

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Our Customers Come First!

We provide quick turnaround time for quotes and formula customization requests to ensure your needs are met efficiently and accurately. We work closely with you to create custom formulas, packaging sizes, and delivery systems that fit your specific requirements and time frames.

About Biologos

Biologos is a full-service manufacturer of standard and custom serum, cell culture media, reagents, and enzyme solutions specializing in quick turn product formulation and distribution.

Manufacturing and Turnkey Expertise: More than 40 years of experience, providing premium quality serum cell culture media, reagents and custom formulations.

Custom Formulation Specialists: Biological solutions customized to your specifications, formulated by Biologos’ experts and delivered on your specified date.

Client Success Team: Personalized and responsive in-house customer service team to assist you with customization, carry out your requests and meet deadlines.

Direct Access to the Manufacturer: Biologos is your manufacturer, warehouse, and distributor, eliminating unnecessary costs and supporting efficiency.

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FAQs About DMEM Media

What Is DMEM Media?

DMEM is a liquid serum-free media and a variant of BME, or Basal Medium Eagle, modified to contain up to four times more vitamins and amino acids, and two to four times more glucose than EMEM. DMEM’s composition makes it an ideal supplement for most cell culture media applications.

However, like EMEM (Essential Modified Eagle’s Medium), DMEM does not contain proteins or agents required to support growth and may need a serum or custom formulation for some cell culture media applications.

dmem media

What Are Common DMEM Media Composition / Modifications?

DMEM (Dulbecco’s Modified Eagle’s Medium) is one of the most customizable biological products available and well suited for a variety of applications. DMEM is manufactured in an aseptic ISO13485 and GMP certified environment starting with a base of inorganic salts, amino acids and vitamins. However, it can be further amended with glucose, HEPES buffer, sodium pyruvate, L-Glutamine and Phenol Red.

DMEM’s composition varies based on the chosen formulation for a specific application. The standard DMEM composition includes four times more vitamins and amino acids than the Basal Medium Eagle medium, from which it is derived. Custom DMEM composition varies and is available upon request from the reagent manufacturer, Biologos.

Why Do We Use DMEM in Cell Culture?

DMEM, like other medias, is used as a synthetic growth medium used in cell culture, with or without added serum, to maintain cells in any environment.

What Are the Uses for Dulbecco’s Modified Eagle’s Medium?

Dulbecco’s Modified Eagle’s Medium, DMEM, is a liquid media supplement used to maintain cells in culture.

How Do You Store and Handle DMEM Media? Is It Safe?

The DMEM Solution is supplied in gamma irradiated, sterile PETG or PETE bottles. We recommend that the DMEM Solution be stored refrigerated at a temperature of 2oC to 8oC. When handling any biological product, it is a best practice to use industrial hygiene and aseptic handling techniques. There are no known hazards with DMEM.

When stored properly, the DMEM can have a shelf life of up to 2 years.

Dulbecco’s Modified Eagle’s Medium (DMEM)

What Is the Difference Between DMEM and EMEM?

DMEM and EMEM are liquid serum-free media formulations and variations of Basal Medium Eagle (BME). Both mediums lack protein and contain the amino acids, salts, glucose and vitamins, required to provide a cell with energy and maintain cells in tissue culture, but the DMEM formulation is modified to contain up to four times more vitamins and amino acids, and two to four times more glucose than EMEM.

This modified composition makes DMEM suitable for more specific cell types.