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Dulbecco’s Phosphate Buffered Saline (DPBS)

Quality and Consistent Phosphate Buffered Saline from A Trusted Biological Manufacturer

Dulbecco’s Phosphate Buffered Saline, DPBS, is water-salt solution used to maintain a consistent pH in biological and chemical applications. It contains disodium hydrogen phosphate and sodium chloride. The pH of standard and custom PBS formulations ranges from 7.2-7.6 when the buffer is ready to use.

DPBS from Biologos is available in multiple formulations and formats to meet your specific research needs, including calcium and magnesium-free. Custom formulations and formulations with custom pH levels are also available.

The Biologos team works directly with you to develop a custom product that meets your specific application requirements. With options like custom packaging, reliable stocking, and tailored logistic plans, Biologos is a perfect partner for every customer.

Media Products

S1221 - PBS, Dulbecco’s Phosphate Buffered Saline (10X) –

S1223 - DPBS, Dulbecco’s Phosphate Buffered Saline (10X) w/o CA & MG

S1220 - PBS, Dulbecco’s Phosphate Buffered Saline (1X) – pH 7

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Our Customers Come First!

We provide quick turnaround time for quotes and formula customization requests to ensure your needs are met efficiently and accurately. We work closely with you to create custom formulas, packaging sizes, and delivery systems that fit your specific requirements and time frames.

About Biologos

Biologos is a full-service manufacturer of standard and custom serum, cell culture media, reagents, and enzyme solutions specializing in quick turn product formulation and distribution.

Manufacturing and Turnkey Expertise: More than 40 years of experience, providing premium quality serum cell culture media, reagents and custom formulations.

Custom Formulation Specialists: Biological solutions customized to your specifications, formulated by Biologos’ experts and delivered on your specified date.

Client Success Team: Personalized and responsive in-house customer service team to assist you with customization, carry out your requests and meet deadlines.

Direct Access to the Manufacturer: Biologos is your manufacturer, warehouse, and distributor, eliminating unnecessary costs and supporting efficiency.

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Call 800-246-4088 or Complete this Form

FAQs About Dulbecco’s Phosphate Buffered Saline (DPBS)

What is DPBS?

Dulbecco’s Phosphate Buffered Saline (DPBS) is intended for use in the maintenance of mammalian cells where a chemically defined, balanced salt solution provides an environment that will maintain the structural and physiological integrity of cells in vitro.


What Are Common DPBS Modification Options (Additives and Concentrations)?

Dulbecco’s Phosphate Buffer Solution is manufactured using inorganic salts and deionized water and can be amended with phenol red (for pH indication) or glucose.

DPBS from Biologos is available with or without calcium and magnesium, as well as, custom formulations and custom pH levels.

What Is the Process of DPBS?

DPBS can be used to maintain a consistent pH in chemical and biological applications. It may also be used as a transport solution or diluent.

Why Do We Use DPBS in Cell Culture?

DPBS is used to maintain cells in a consistent PH so that do not expand or contract. Within tissue processing specifically, it can be used to clean cells, or used in combination with other products to preserve cells that are grown or harvested from a donor.

Does DPBS Follow Any Regulations or Standards?

All Biologos DPBS is manufactured in an ISO13485 and GMP certified environment.

What Are the Uses for DPBS?

DPBS is mostly commonly used as buffer solution in biological and chemical applications, but can be formulated with additives for use in other applications, such as:

  • A diluent for concentrated substances
  • A rinsing agent, for containers with cells
  • Separating clumped cells (PBS with EDTA)

The product is intended to be used in vitro, in laboratory only. Do not use it in therapy, human or veterinary applications.

How Do You Store and Transport DPBS?

The DPBS is supplied in gamma irradiated, sterile PETG or PETE bottles. We recommend that the DPBS should be at ambient temperatures. Always use aseptic techniques when handling DPBS.

The DPBS is shipped ambient by less than truck-load carrier or overnight, as needed.

How Do You Handle DPBS? Is It Safe?

When handling all biological products, it is a best practice to use industrial hygiene standards and aseptic handling techniques. If ingested, inhaled or in contact with skin, DPBS can cause tissue irritation.

Dulbecco's Phosphate Buffered Saline