Premium Quality Serum, Cell Culture Media & Reagents

Premium Quality Serum, Cell Culture Media & Reagents

Dulbecco's Phosphate-Buffered Saline (DPBS)

Product Information

Dulbecco’s Phosphate Buffered Saline, shortened to DPBS, is water-salt solution used to maintain a consistent pH in biological and chemical applications. It contains disodium hydrogen phosphate and sodium chloride. The pH of standard and custom PBS formulations ranges from 7.2-7.6 when the buffer is ready to use.

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Modification Options

  • W, W/O Calcium and Magnesium
  • W, W/O NaHCO3
  • Custom Formulations Available
Testing: pH, Osmolality, Sterility for 9CFR or USP


Product Type: DPBS
Color: Clear, Colorless Solution
pH: 7.0 - 7.4
Form: Solution

Packaging Options

  • 500 mL
  • 1000 mL
  • 3000 mL
  • Custom Packaging Available

Shipping and Storage

  • Storage & Shipping: Ambient
  • Shelf Life: 24 Months


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Dulbecco's Phosphate-Buffered Saline (DPBS) Catalog

Product Number Product Name Spec Sheet Safety Data Sheet
S12203 DPBS (1X) – pH 7.4 Download N/A
S12202 DPBS(1X) w/o NaHCO3 Download Download
S1222 DPBS (1X) w/o Ca & Mg Download Download
S1221 DPBS (10X) Download N/A
S1223 DPBS (10X) w/o CA & MG Download N/A
S1220 DPBS (1X) – pH 7 Download N/A

What Is the Difference Between PBS and DPBS? Is There a Difference Between PBS and DPBS?


Phosphate Buffer Solution = Phosphate Buffer. Both PBS & DPBS have uses in maintaining pH constants, as well as irrigation or transport use in tissue processing. Dulbecco’s differs with a range of concentrations (ex. 1X, 10X), and additives, such as Calcium and Magnesium.

PBS and DPBS can often be used interchangeably because they provide the same functions and have similar composition; however, DPBS has a lower concentration of phosphate.


Is DPBS Regulated Or Have Government Standards Or Specifications?


All Biologos DPBS is manufactured in a ISO13485 and GMP certified environment. 


Why Do We Use DPBS in Cell Culture?


DPBS is used to maintain cells in a consistent pH so that they do not expand or contract. Within tissue processing specifically, it can be used to clean cells, or could be used in combination with other products to preserve cells that are grown or harvested from a donor. 


How to Handle DPBS? Is it Safe?


When handling all biological products, it is a best practice to use industrial hygiene standards and aseptic handling techniques. If ingested, inhaled or in contact with skin, DPBS can cause tissue irritation. 


How Does DPBS Work?


DPBS can be used to maintain a consistent pH in chemical and biological applications. 

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