Fetal Bovine Serum (FBS)

Fetal Bovine Serum (FBS)

Description:

Fetal Bovine Serum (FBS) is the most commonly used media supplement for cells in culture. Biologos supplies FBS lots with excellent cell growth characteristics, low endotoxin and low hemoglobin values.

Collection and Processing:

Origin:

The FBS Serum used by Biologos meets all of the USDA requirements for animal products. All FBS Serum is traceable back to the date and location of collection. In addition, all of the FBS Serum used for processing must meet Biologos’ strict quality requirements for raw material.

Filtration:

Approved lots of raw FBS Serum are thawed under controlled conditions and sterile filtered by a process that uses a series of filters in descending pore size, utilizing 0.1 micron filters for the final filtration step. Filling takes place in a laminar flow hood and clean room certified to maintain ISO Class 5 conditions. The FBS Serum is aseptically dispensed into gamma irradiated, sterile PETG or HDPE bottles. Filled containers are labeled, frozen, and then maintained at temperatures less than -5oC to preserve the product quality.

Quality Control Testing

Microbiological Testing:

Each lot of FBS Serum is tested to confirm the absence of bacterial or fungal contamination. Each lot of FBS Serum is also tested to confirm the absence of mycoplasma contamination.

Virus Testing:

The FBS Serum is additionally tested for adventitious agents using modified procedures adapted from the Code of Federal Regulations, Title 9, Section 113.53, “Requirements for Ingredients of Animal Origin”.

Storage and Handling

The FBS Serum is shipped in gamma irradiated, sterile PETG or HDPE bottles. Biologos recommends that the FBS Serum be stored frozen at a temperature of -5oC to -20oC. Multiple freeze-thaw cycles of the serum should be avoided as this may lead to deterioration of the product.

Shipping

FBS Serum is shipped frozen by freezer truck or next day air in insulated containers packed with dry ice.

What is Fetal Bovine Serum?

Fetal Bovine Serum (FBS) is the clear, orange fluid that is obtained from separating blood, sourced from a bovine fetus, in to its solid and liquid components. Fetal Bovine Serum is among most widely used serum-supplement for cells in culture. FBS has a very low level of antibodies and contains more growth factors than other sera, this allows for versatility in different cell culture applications.

Source

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