Minimum Essential Medium (MEM)

Product Overview


Minimum Essential Medium (MEM) is a modified formulation of Basal Medium Eagle media with a higher concentration of essential nutrients. Minimum Essential Medium is widely used for a variety of adherent and suspension mammalian cells, that was developed by Harry Eagle when Basal Medium Eagle (BME) was found to be inadequate to support the growth of HeLa cells.

Formulation can be incorporated to include either Hank’s or Earle’s salt formulations.

  • Optional supplementation of non-essential amino acids (NEAA)
  • Optional supplementation of HEPES buffer
  • Can be manufactured with or without L-Glutamine
  • Can be formulated to optimized specifically for suspension culture

Additional Information:

What is MEM?
MEM Storage and Handling
MEM Uses

What is MEM?

Minimum Essential Medium (MEM) is a modified formulation of Basal Medium Eagle (BME) media with a higher concentration of essential nutrients than BME.

MEM Storage and Handling

MEM is supplied in gamma irradiated, sterile PETG or PETE bottles. We recommend that the MEM  be stored refrigerated at a temperature of 2C to 8C. MEM has a shelf life of 18-24 months depending on formulation.

MEM is shipped ambient ground carrier or overnight, as requested by the customer.

MEM Uses

Minimum Essential Medium is widely used for a variety of adherent and suspension mammalian cells.

Formulation can be incorporated to include either Hank’s or Earle’s salt formulations.

  • Optional supplementation of non-essential amino acids (NEAA)
  • Optional supplementation of HEPES buffer
  • Can be manufactured with or without L-Glutamine
  • Can be formulated to optimized specifically for suspension culture

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Media, Media Featured Products


LMH9010 - MEM, Minimum Essential Medium (Eagle) w/Hanks’ Salts, HEPES Buffer & L-Glutamine; w/o Sodium Pyruvate

LMH9011 - MEM, Minimum Essential Medium (Eagle) w/Hanks’ Salts, HEPES Buffer, L-Glutamine & Sodium Pyruvate

LMH2010 - MEM, Minimum Essential Medium (Eagle) w/Hanks’ Salts & L-Glutamine; w/o Sodium Pyruvate & Sodium Bicarbonate

LMH2011 - MEM, Minimum Essential Medium (Eagle) w/Hanks’ Salts, L-Glutamine & Sodium Pyruvate; w/o Sodium Bicarbonate

LM0101 - MEM, Minimum Essential Medium (Eagle) – Alpha, w/Sodium Pyruvate; w/o L-Glutamine


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