Minimum Essential Medium (MEM)

Product Overview


Premium quality, serum-free media for maintaining cells

Minimum Essential Medium (MEM) is a is a synthetic cell culture medium and variant of Basal Medium Eagle (BME), modified to contain 2x more amino acids. This composition is similar to the protein composition in human cells, allowing cultured human cells to be maintained for longer periods of time without repeat feedings.

Formulations can be incorporated to include either Hank’s or Earle’s salt formulations.

  • Optional supplementation of non-essential amino acids (NEAA)
  • Optional supplementation of HEPES buffer
  • Can be manufactured with or without L-Glutamine
  • Can be formulated to optimized specifically for suspension culture

Media Products


LMH9010 - MEM, Minimum Essential Medium (Eagle) w/Hanks’ Salts, HEPES Buffer & L-Glutamine; w/o Sodium Pyruvate

LMH9011 - MEM, Minimum Essential Medium (Eagle) w/Hanks’ Salts, HEPES Buffer, L-Glutamine & Sodium Pyruvate

LMH2010 - MEM, Minimum Essential Medium (Eagle) w/Hanks’ Salts & L-Glutamine; w/o Sodium Pyruvate & Sodium Bicarbonate



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About Biologos

Biologos is a full-service manufacturer of standard and custom serum, cell culture media, reagents, and enzyme solutions specializing in quick turn product formulation and distribution.

Manufacturing and Turnkey Expertise: More than 40 years of experience, providing premium quality serum cell culture media, reagents and custom formulations.

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About MEM Media

What is MEM?

Minimum Essential Medium (MEM) is a synthetic cell culture medium and variant of variant of Basal Medium Eagle (BME), modified to contain 2x more amino acids, used for cultured mammalian cells.

MEM Storage and Handling

MEM is supplied in gamma irradiated, sterile PETG or PETE bottles. We recommend that the MEM  be stored refrigerated at a temperature of 2C to 8C. MEM has a shelf life of 18-24 months depending on formulation.

MEM is shipped ambient ground carrier or overnight, as requested by the customer.

The Difference Between DMEM and MEM

DMEM is a liquid serum-free media and a variant of BME, or Basal Medium Eagle, modified to contain up to four times more vitamins and amino acids, and two to four times more glucose than EMEM. Additional components in DMEM's composition are glycine, serine and ferric nitrate.

Neither DMEM nor EMEM contain proteins or agents required to support growth and may require a custom formulation for some cell culture media applications.

MEM Composition

Standard EMEM media contains balanced salt solution, glucose, vitamins, 2x more amino acids than its derivative, Basal Medium Eagle (BME) and sodium pyruvate. Custom EMEM composition varies and is available upon request from the reagent manufacturer, Biologos. To request the composition of a custom EMEM formulation, email: [email protected]

MEM Uses

Minimum Essential Medium is widely used for a variety of adherent and suspension mammalian cells.

Formulation can be incorporated to include either Hank’s or Earle’s salt formulations.

  • Optional supplementation of non-essential amino acids (NEAA)
  • Optional supplementation of HEPES buffer
  • Can be manufactured with or without L-Glutamine
  • Can be formulated to optimized specifically for suspension culture