Menu Close

Phosphate Buffered Saline (PBS)

Quality and Consistent Phosphate Buffered Saline from A Trusted Biological Manufacturer

Phosphate Buffered Saline is water-salt solution used to maintain a consistent pH in biological and chemical applications. It contains disodium hydrogen phosphate, sodium chloride. The pH of standard and custom PBS formulations range from 7-7.4 when the buffer is ready to use.

PBS from Biologos is available in 1x or 10x concentrations, with or without calcium and magnesium, as well as, custom formulations and custom pH levels.

The Biologos team works directly with you to develop a custom product that meets your specific application requirements. With options like custom packaging, reliable stocking, and tailored logistic plans, Biologos is a perfect partner for every customer.

Request A Quote / More Information

Our Customers Come First!

We provide quick turnaround time for quotes and formula customization requests to ensure your needs are met efficiently and accurately. We work closely with you to create custom formulas, packaging sizes, and delivery systems that fit your specific requirements and time frames.


About Biologos

Biologos is a full-service manufacturer of standard and custom serum, cell culture media, reagents, and enzyme solutions specializing in quick turn product formulation and distribution.

Manufacturing and Turnkey Expertise: More than 40 years of experience, providing premium quality serum cell culture media, reagents and custom formulations.

Custom Formulation Specialists: Biological solutions customized to your specifications, formulated by Biologos’ experts and delivered on your specified date.

Client Success Team: Personalized and responsive in-house customer service team to assist you with customization, carry out your requests and meet deadlines.

Direct Access to the Manufacturer: Biologos is your manufacturer, warehouse, and distributor, eliminating unnecessary costs and supporting efficiency.

Request A Quote

Call 800-246-4088 or Complete this Form

FAQs About PBS Media

What is PBS/ PBS Biology?

Phosphate Buffered Saline, PBS, is a water-based salt solution containing sodium chloride and sodium phosphate. PBS is commonly formulated to help cells maintain a constant pH and osmosis but can be modified for use in other applications related to biological research.

What Are the Different Varieties of PBS, if Any?

Phosphate Buffer solution is manufactured with a R.O.D.I water and USP grade sodium phosphate solutions. The concentration of R.O.D.I water, monobasic & dibasic solutions can be customized as desired by customer.

What Are the Uses of PBS?

PBS is mostly commonly used as buffer solution in biological and chemical applications, but can be formulated with additives for use in other applications, such as:

  • A diluent for concentrated substances
  • A rinsing agent, for containers with cells
  • Separating clumped cells (PBS with EDTA)

How Do You Store and Transport PBS? What Is the Normal Shelf Life?

Phosphate Buffered Saline is supplied in gamma-irradiated, sterile PETG or PETE bottles. We recommend that PBS solution be stored at ambient temperatures.

When transporting, PBS is shipped ambient by less than truck-load carrier or overnight, as needed.

The shelf life of phosphate-buffered saline is of 2 years when stored properly.

How Do You Handle PBS?

When handling all biological products, it is a best practice to use industrial hygiene standards and aseptic handling techniques. If PBS comes in contact with the eye, irritation is likely.

Why Do We Wash Cells With PBS?

As PBS’ osmolarity and ion concentrations closely match the human body, PBS is non-toxic to cells.

What Are the Formulation Options? What Can Be Added to the Base Formula?

Biologos manufactures three standard formulizations of phosphate buffer solution, each with a different monobasic & dibasic sodium phosphate solution mix, all of which are commonly used in tissue recovery. Further custom formulizations, typically using potassium chloride and/or potassium dihydrogen phosphate, are also available.

What Are Common PBS Composition / Modifications?

Basic Phosphate Buffered Saline, or PBS consists of sodium chloride and disodium hydrogen phosphate. Common additives are potassium chloride or potassium phosphate. PBS from Biologos is available in 1x or 10x concentrations, with or without calcium and magnesium, as well as, custom formulations and custom pH levels.

Biologos makes each PBS formulation upon request. After your order is placed, we will stock and store your custom PBS formulations in our GMP certified facility and ship your formulation as you request it- ensuring product quality.

What Is the Difference Between PBS, Phosphate Buffer, and DPBS? Is There a Difference?

Phosphate Buffer Solution = Phosphate Buffer. Both PBS & DPBS have uses in maintaining pH constants, as well as irrigation or transport use in tissue processing. Dulbecco’s differs with a range of concentrations (ex. 1X, 10X), and additives, such as Calcium and Magnesium.

PBS and DPBS can often be used interchangeably because they provide the same functions and have similar composition; however, DPBS has a lower concentration of phosphate.