Phosphate Buffered Saline (PBS)

Formulations of PBS without calcium or magnesium are made due to a requirement to rinse chelators from the culture before dissociation of the cells. 

Summary:

DPBS and PBS are actually more similar than they are different. PBS and DPBS can often be used interchangeably because they provide the same functions and have similar composition. Many believe the formulas are different due to the difference in their names; however, the only difference is within their customization. When choosing a formula to customize, DPBS provides are more defined starting formula over PBS which is a broader formulation to start from.

What is Dulbecco’s Phosphate Buffer Saline (DPBS)?

Dulbecco’s Phosphate Buffered Saline, shortened to DPBS, is water-salt solution used to maintain a consistent pH in biological and chemical applications.

What is Phosphate Buffer Saline (PBS)?

Phosphate Buffered Saline, PBS, is a water-based salt solution containing sodium chloride and sodium phosphate.

Does BIOLOGOS Prepare Phosphate Buffer Saline (PBS) & Dulbecco’s Phosphate Buffer Saline (DPBS)?

The BIOLOGOS team supports your urgent DPBS and PBS needs. As a BIOLOGOS customer, your standard and custom DPBS or PBS is stocked, stored and ready to ship based on your needs and on your schedule.

Basic Phosphate Buffered Saline, or PBS consists of sodium chloride and disodium hydrogen phosphate. Common additives are potassium chloride or potassium phosphate. PBS from BIOLOGOS is available in 1x or 10x concentrations, with or without calcium and magnesium, as well as, custom formulations and custom pH levels.

BIOLOGOS makes each PBS formulation upon request. After your order is placed, we will stock and store your custom PBS formulations in our GMP certified facility and ship your formulation as you request it- ensuring product quality.

BIOLOGOS manufactures three standard formulizations of phosphate buffer solution, each with a different monobasic & dibasic sodium phosphate solution mix, all of which are commonly used in tissue recovery. Further custom formulizations, typically using potassium chloride and/or potassium dihydrogen phosphate, are also available.

As PBS’ osmolarity and ion concentrations closely match the human body, PBS is non-toxic to cells.

When handling all biological products, it is a best practice to use industrial hygiene standards and aseptic handling techniques. If PBS comes in contact with the eye, irritation is likely.

Phosphate Buffered Saline is supplied in gamma-irradiated, sterile PETG or PETE bottles. We recommend that PBS solution be stored at ambient temperatures.

When transporting, PBS is shipped ambient by less than truck-load carrier or overnight, as needed.

The shelf life of phosphate-buffered saline is of 2 years when stored properly.

PBS is mostly commonly used as buffer solution in biological and chemical applications, but can be formulated with additives for use in other applications, such as:

• A diluent for concentrated substances
• A rinsing agent, for containers with cells
• Separating clumped cells (PBS with EDTA)

Phosphate Buffer solution is manufactured with a R.O.D.I water and USP grade sodium phosphate solutions. The concentration of R.O.D.I water, monobasic & dibasic solutions can be customized as desired by customer.

Phosphate Buffered Saline, PBS, is a water-based salt solution containing sodium chloride and sodium phosphate. PBS is commonly formulated to help cells maintain a constant pH and osmosis but can be modified for use in other applications related to biological research.