RPMI 1640

Product Overview


RPMI 1640 is an all-purpose media that supports the growth of a wide variety of cells in suspension and grown as monolayers.

If properly supplemented with serum, adequate serum replacement, or proper growth factor supplementation, RPMI 1640 has a wide range of applications for mammalian cells, including the culture of fresh human lymphocytes, fusion protocols, growth of hybrid cells and hematopoietic cells.

RPMI 1640 was developed at Roswell Park Memorial Institute (RPMI) in Buffalo, New York. RPMI 1640 is a modification of McCoy’s 5A and was developed for the long-term culture of peripheral blood lymphocytes.

RPMI 1640 can be manufactured with or without:
  • 25 mM HEPES buffer
  • L-Glutamine
  • Phenol Red
  • Sodium Bicarbonate

Media Products


LR010 - RPMI 1640 (1X) w/L-Glutamine; w/o Phenol Red

LR911 - RPMI 1640(1X) W/ HEPES

LR311 - RPMI 1640(1X) with 10% Glycerine

LRX11 - RPMI 1640(10X)



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About RPMI 1640 Media

What is RPMI 1640?

RPMI 1640 is an all-purpose media with a broad range of applications for mammalian cells.

RPMI 1640 Customizations

RPMI 1640 can be manufactured with or without:
  • 25 mM HEPES buffer
  • L-Glutamine
  • Phenol Red
  • Sodium Bicarbonate

RPMI 1640 from Biologos can be packaged according to your specifications, 1000 ml and 500 ml fill sizes available.

RPMI 1640 Storage and Handling

RPMI 1640 Solution is supplied in gamma irradiated, sterile PETG or PETE bottles. We recommend that the RPMI 1640 Solution be stored refrigerated at a temperature of 2C to 8C. RPMI 1640 has a shelf life of 18-24 months depending on formulation.

RPMI 1640 is shipped ambient ground carrier or overnight, as requested by the customer.

The shelf life of RPMI 1640, when stored properly, 18 -24 months depending on formulation.

RPMI 1640 Uses

RPMI 1640 has a wide range of applications for mammalian cells, including the culture of fresh human lymphocytes, fusion protocols, growth of hybrid cells and hematopoietic cells.

RPMI 1640 was developed at Roswell Park Memorial Institute (RPMI) in Buffalo, New York. RPMI 1640 is a modification of McCoy’s 5A and was developed for the long-term culture of peripheral blood lymphocytes.