RPMI-1640 supports the growth of a wide variety of cells in suspension and grown as monolayers.
If properly supplemented with serum, adequate serum replacement, or proper growth factor supplementation, RPMI-1640 has a wide range of applications for mammalian cells, including the culture of fresh human lymphocytes, fusion protocols, growth of hybrid cells and hematopoietic cells.
Storage and Handling
The RPMI 1640 Solution is supplied in gamma irradiated, sterile PETG or PETE bottles. We recommend that the RPMI 1640 Solution be stored refrigerated at a temperature of 2C to 8C. RPMI 1640 has a shelf life of 18-24 months depending on formulation.