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RPMI 1640

Product Information

RPMI 1640 is an all-purpose media that supports the growth of a wide variety of cells in suspension and grown as monolayers. If properly supplemented with serum, adequate serum replacement, or proper growth factor supplementation, RPMI 1640 has a wide range of applications for mammalian cells, including the culture of fresh human lymphocytes, fusion protocols, growth of hybrid cells and hematopoietic cells.

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Modification Options

  • With/Without HEPES Buffer
  • L-Glutamine
  • Sodium Pyruvate
  • Phenol Red
  • Custom Formulations Available
Testing: Sterility, Osmolality, pH

Specifications

Product Type: RPMI 1640
Color: Clear, Red Solution
Concentration: 1X
pH: 7.0 - 7.4
Form: Solution

Packaging Options

  • 500 mL
  • 1000 mL
  • 3000 mL
  • Custom Packaging Available

Shipping and Storage

  • Storage & Shipping: Refrigerated
  • Shelf Life: 18 Months

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RPMI 1640 Catalog

Product Number Product Name Spec Sheet Safety Data Sheet
LR311 RPMI 1640 (1X) with 10% Glycerine Download Download
LR911 RPMI 1640 (1X) W/ HEPES Download Download
LR010 RPMI 1640 (1X) w/L-Glutamine; w/o Phenol Red N/A N/A
LR910 RPMI 1640 (1X) w/HEPES Buffer & L-Glutamine; w/o Phenol Red Download Download
LR011 RPMI 1640 (1X) Download Download
Q:

What Does RPMI Stand For?

A:

RPMI 1640 was developed at Roswell Park Memorial Institute (RPMI) in 1966 as a modification of McCoy’s 5A (RPMI-1630).

Q:

What Is the Difference Between RPMI and DMEM?

A:

Both RPMI-1640 and DMEM are both classical basal medias, but each have specific cell lines that they best support.

Q:

What Are the Uses for RPMI 1640?

A:

RPMI 1640 has a wide range of applications for mammalian cells, including the culture of fresh human lymphocytes, fusion protocols, growth of hybrid cells and hematopoietic cells. RPMI 1640 was developed at Roswell Park Memorial Institute (RPMI) in Buffalo, New York, and is a modification of McCoy’s 5A and was developed for the long-term culture of peripheral blood lymphocytes.

Q:

How Do You Store and Handle RPMI 1640?

A:

RPMI 1640 Solution is supplied in gamma irradiated, sterile PETG or PETE bottles. We recommend that the RPMI 1640 Solution be stored refrigerated at a temperature of 2C to 8C. RPMI 1640 has a shelf life of 18-24 months, depending on formulation.

Q:

What Are the Customization Options of RPMI 1640, if Any?

A:

RPMI 1640 can be manufactured with or without 25mM HEPES buffer, high/low glucose, L-Glutamine, Phenol Red, Sodium Bicarbonate, Sodium Pyruvate and many other modifications.

Q:

What is RPMI 1640?

A:

RPMI 1640 is an all-purpose media with a broad range of applications for mammalian cells.

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