Menu Close

Trypsin (1X)

Quality and Consistent Trypsin Formulations from A Trusted Biological Manufacturer

Often used in proteomic research, Trypsin is an enzyme that breaks down proteins to separate or detach cells from the culture vessel. This process is important when cells need to move from vessel to vessel, and when complete, the cells will become suspended and appear rounded. Biologos produces quality Trypsin, formulated to perform consistently in every application. 

The Biologos team works directly with you to develop a custom product that meets your specific application requirements. With options like custom packaging, reliable stocking, and tailored logistic plans, Biologos is a perfect partner for every customer.

Media Products

E1510 - E1510 – Trypsin EDTA Solution (10X) in HBSS Solution

E1500 - Trypsin Solution 1.0%

E1501 - Trypsin Solution O.25%

E1502 - Trypsin Solution 2.5% (1:250)

Request A Quote / More Information

Our Customers Come First!

We provide quick turnaround time for quotes and formula customization requests to ensure your needs are met efficiently and accurately. We work closely with you to create custom formulas, packaging sizes, and delivery systems that fit your specific requirements and time frames.

About Biologos

Biologos is a full-service manufacturer of standard and custom serum, cell culture media, reagents, and enzyme solutions specializing in quick turn product formulation and distribution.

Manufacturing and Turnkey Expertise: More than 40 years of experience, providing premium quality serum cell culture media, reagents and custom formulations.

Custom Formulation Specialists: Biological solutions customized to your specifications, formulated by Biologos’ experts and delivered on your specified date.

Client Success Team: Personalized and responsive in-house customer service team to assist you with customization, carry out your requests and meet deadlines.

Direct Access to the Manufacturer: Biologos is your manufacturer, warehouse, and distributor, eliminating unnecessary costs and supporting efficiency.

Request A Quote

Call 800-246-4088 or Complete this Form

FAQs About Trypsin (1X)

What is Trypsin?

Trypsin is a proteolytic enzyme produced by the pancreas to breakdown proteins into amino acids for digestion and other biological processes. The pH and temperature of Trypsin affect its ability to function, pH is determined by the temperature of the solution and formulation. Trypsin in an inactive state has a pH of 3 and in an active state has a pH of 8.

What Are Common Trypsin Enzyme Formulation Options (Additives and Concentrations)?

Biologos offers Trypsin enzyme products in multiple formulations and concentrations (see below), customizable container sizes (100 ml, 500 ml, 1 Liter, 20 Liter, etc.) and varied container types (sterile bottles, sterile bags, etc.). Based on the application and cell types, trypsin is produced with various constituents and at various concentration. The most common variations are:

  • EDTA (disodium ethylenediaminetetraacetic acid) – To weaken interactions, EDTA is frequently included with Trypsin which chelates the divalent cations (Ca2+, Mg 2+).
  • Phenol red – Optimum activity of trypsin is achieved within the pH range of 7 to 9. The inclusion of phenol red within the specified pH range, gives the solution its pink color.
  • Diluent – Concentrated trypsin is solubilized or diluted in a buffered salt solution that contains no Ca2+ or Mg2+, such as Hank’s Balanced Salt Solution, which maintains pH and osmotic balance. Some trypsin products have 0.9% NaCL as diluent instead of Hank’s Balanced Salt Solution.
  • Concentration – Based on the cell type and application trypsin is used in various concentrations. For strongly adherent cell lines, trypsin of 2.5 % to 0.25% (10X to 1X power) is used. For studies requiring the integrity of cell surface proteins, lower concentrated (0.05% trypsin) solutions are employed.
Trypsin or Trypsin Enzyme

Why Is Trypsin Used in Cell Culture?

Within the human & animal health pharma worlds, Trypsin can be used in research to convert proteins into peptides.

What Are the Uses for Trypsin?

Trypsin has many effectives uses and is often used in the animal health & Human pharma worlds for microbiological media and cell harvesting. Trypsin is commonly used to develop cell and tissue culture protocols and identify proteins, but can be formulated with inhibitors or activators for use in other applications, such as:

  • Cell harvesting
  • Tissue dissociation
  • Hemagglutination procedures
  • Reducing cell density in cell culture
  • Subculturing cells

How Do You Prepare Trypsin?

Trypsin is manufactured in an aseptic environment and tested for 9 CFR 113.26 Sterility. Raw Trypsin powder is screened for porcine viruses and mycoplasma per 9 CFR 113.53c.

How Do You Store and Transport Trypsin?

Trypsin products are supplied in gamma irradiated, sterile PETG or PETE bottles and are available in customizable sterile LDPE bags. Biologos recommends that Trypsin products be stored frozen at a temperature range of -50C to -100C.

Trypsin products are shipped frozen by next day air in temperature-controlled LTL freight delivery trucks.

How Do You Handle Trypsin? Is It Safe?

There are no known hazards with Trypsin products, but it is always best to use industrial hygiene measures when in contact with any biological product and to handle product using aseptic techniques.