Trypsin is a proteolytic enzyme that is used to detach cells from an adherent substrate. It is a member of serine protease family and is most frequently used. Trypsin is produced from proenzyme, trypsinogen secreted by exocrine cells of pancreas; Trypsin acts on C-terminal side of Lysine or Arginine. Long term incubation with high trypsin concentration damages cells by striping cell surface proteins and kill the cells.
Biologos offers Trypsin products in multiple formulations and concentrations (see below), customizable container sizes (100 ml, 500 ml, 1 Liter, 20 Liter, etc.) and varied container types (sterile bottles, sterile bags, etc.). Based on the application and cell types, trypsin is produced with various constituents and at various concentration. Some options listed below:
Trypsin products are supplied in gamma irradiated, sterile PETG or PETE bottles and in customizable sterile LDPE bags. Biologos recommends that Trypsin products be stored frozen at a temperature range of -50C to -100C. Always use aseptic techniques when handling the Trypsin Solutions.
Trypsin products are shipped frozen by next day air in temperature-controlled LTL freight delivery trucks.
Trypsin is a proteolytic enzyme that is used to detach cells from an adherent substrate. It is a member of serine protease family and is most frequently used.