Trypsin (1X)

Quality and Consistent Trypsin Formulations from A Trusted Biological Manufacturer


Trypsin is a proteolytic enzyme produced by the pancreas to breakdown proteins into amino acids for digestion and other biological processes. The pH and temperature of Trypsin affect its ability to function, pH is determined by the temperature of the solution and formulation. Trypsin in an inactive state has a pH of 3 and in an active state has a pH of 8.

The Biologos team works directly with you to develop a custom product that meets your specific application requirements. With options like custom packaging, reliable stocking, and tailored logistic plans, Biologos is a perfect partner for every customer.

Enzyme Solutions Products


E1507 - Trypsin Solution 2.5% (1X) in HBSS, w/o Calcium & Magnesium

E1506 - Trypsin – EDTA 0.25% Solution in HBSS, w/o Calcium & Magnesium

E15032 - Trypsin (0.5 g/L)-EDTA (0.2 g/L) Solution (1X) in Normal Saline

E1505 - Trypsin-EDTA Soln.(1x) in HBSS

E1504 - Trypsin-EDTA Sol. ( 10X )



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We provide quick turnaround time for quotes and formula customization requests to ensure your needs are met efficiently and accurately. We work closely with you to create custom formulas, packaging sizes, and delivery systems that fit your specific requirements and time frames.


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About Trypsin (1X)

What is Trypsin?

Trypsin is a proteolytic enzyme produced by the pancreas to breakdown proteins into amino acids for digestion and other biological processes. The pH and temperature of Trypsin affect its ability to function, pH is determined by the temperature of the solution and formulation. Trypsin in an inactive state has a pH of 3 and in an active state has a pH of 8.

Trypsin Storage and Handling

Trypsin products are supplied in gamma irradiated, sterile PETG or PETE bottles and are available in customizable sterile LDPE bags. Biologos recommends that Trypsin products be stored frozen at a temperature range of -50C to -100C. Always use aseptic techniques when handling the Trypsin Solutions.

Trypsin products are shipped frozen by next day air in temperature-controlled LTL freight delivery trucks.

Trypsin Uses

Trypsin is commonly used to develop cell and tissue culture protocols and identify proteins, but can be formulated with inhibitors or activators for use in other applications, such as:

  • Cell harvesting
  • Tissue dissociation
  • Hemagglutination procedures
  • Reducing cell density in cell culture
  • Subculturing cells

Trypsin Formulation Options(Additives and Concentrations)

Biologos offers Trypsin products in multiple formulations and concentrations (see below), customizable container sizes (100 ml, 500 ml, 1 Liter, 20 Liter, etc.) and varied container types (sterile bottles, sterile bags, etc.). Based on the application and cell types, trypsin is produced with various constituents and at various concentration. The most common variations are:
  • EDTA (disodium ethylenediaminetetraacetic acid) - To weaken interactions, EDTA is frequently included with Trypsin which chelates the divalent cations (Ca2+, Mg 2+).
  • Phenol red - Optimum activity of trypsin is achieved within the pH range of 7 to 9. The inclusion of phenol red within the specified pH range, gives the solution its pink color.
  • Diluent - Concentrated trypsin is solubilized or diluted in a buffered salt solution that contains no Ca2+ or Mg2+, such as Hank's Balanced Salt Solution, which maintains pH and osmotic balance. Some trypsin products have 0.9% NaCL as diluent instead of Hank's Balanced Salt Solution.
  • Concentration - Based on the cell type and application trypsin is used in various concentrations. For strongly adherent cell lines, trypsin of 2.5 % to 0.25% (10X to 1X power) is used. For studies requiring the integrity of cell surface proteins, lower concentrated (0.05% trypsin) solutions are employed.