Trypsin (1X)

Trypsin: Proteolytic Enzyme

Information and Uses

Trypsin is a proteolytic enzyme that is used to detach cells from an adherent substrate.  It is a member of serine protease family and is most frequently used.  Trypsin is produced from proenzyme, trypsinogen secreted by exocrine cells of pancreas; Trypsin acts on C-terminal side of Lysine or Arginine.  Long term incubation with high trypsin concentration damages cells by striping cell surface proteins and kill the cells.

Formulations

Biologos offers Trypsin products in multiple formulations and concentrations (see below), customizable container sizes (100 ml, 500 ml, 1 Liter, 20 Liter, etc.) and varied container types (sterile bottles, sterile bags, etc.).  Based on the application and cell types, trypsin is produced with various constituents and at various concentration.  Some options listed below:

  • EDTA (disodium ethylenediaminetetraacetic acid) – To weaken the interactions, EDTA is frequently included with Trypsin which chelates the divalent cations (Ca2+, Mg 2+).
  • Phenol red – Optimum activity of trypsin is achieved at pH range from 7 to 9. At this range inclusion of phenol red gives pink color.
  • Diluent – Concentrated trypsin is being solubilized or diluted in a buffered salt solution that contains no Ca2+ or Mg2+, such as Hank’s Balanced Salt Solution, which maintains pH and osmotic balance. Some trypsin products have 0.9% NaCL as diluent instead of hanks balanced salt solution.
  • Concentration – Based on the cell type and application trypsin is used in various concentrations. For strongly adherent cell lines, trypsin of 2.5 % to 0.25% (10X to 1X power) is used. For studies which require cell surface protein integrity, lower concentrated (0.05% trypsin) solutions are employed.

Storage and Handling

Trypsin products are supplied in gamma irradiated, sterile PETG or PETE bottles and in customizable sterile LDPE bags. Biologos recommends that Trypsin products be stored frozen at a temperature range of -50C to -100C. Always use aseptic techniques when handling the Trypsin Solutions.

Shipping

Trypsin products are shipped frozen by next day air in temperature-controlled LTL freight delivery trucks.

Industry Uses for Trypsin

Trypsin is a proteolytic enzyme that is used to detach cells from an adherent substrate.  It is a member of serine protease family and is most frequently used.

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