Trypsin

People that search for Trypsin from BIOLOGOS also consider these Trypsin products: Thermofisher 25200056 100mL, ThermoFisher 25200072 500mL, Millipore Sigma 59418C-100ML, Corning 25-052-CI 100mL, Corning 25-052-CV 500 mL, VWR 45001-082 100mL and VWR 45000-662 500mL. If you have a specific formulation request, contact BIOLOGOS directly. We’re happy to help you find the right product for you!

There are no known hazards with Trypsin products, but it is always best to use industrial hygiene measures when in contact with any biological product and to handle product using aseptic techniques.

Trypsin products are supplied in gamma irradiated, sterile PETG or PETE bottles and are available in customizable sterile LDPE bags. BIOLOGOS recommends that Trypsin products be stored frozen at a temperature range of -50C to -100C.

Trypsin products are shipped frozen by next day air in temperature-controlled LTL freight delivery trucks.

Trypsin is manufactured in an aseptic environment and tested for 9 CFR 113.26 Sterility. Raw Trypsin powder is screened for porcine viruses and mycoplasma per 9 CFR 113.53c.

Trypsin has many effective uses and is often used in the animal health & human pharma worlds for microbiological media and cell harvesting. Trypsin is commonly used to develop cell and tissue culture protocols and identify proteins, but can be formulated with inhibitors or activators for use in other applications, such as:

• Cell harvesting
• Tissue dissociation
• Hemagglutination procedures
• Reducing cell density in cell culture
• Subculturing cells

Within the human & animal health pharma worlds, Trypsin can be used in research to convert proteins into peptides.

BIOLOGOS offers Trypsin enzyme products in multiple formulations and concentrations (see below), customizable container sizes (100 ml, 500 ml, 1 Liter, 20 Liter, etc.) and varied container types (sterile bottles, sterile bags, etc.). Based on the application and cell types, trypsin is produced with various constituents and at various concentration. The most common variations are:

• EDTA (disodium ethylenediaminetetraacetic acid) – To weaken interactions, EDTA is frequently included with Trypsin which chelates the divalent cations (Ca2+, Mg 2+).
• Phenol red – Optimum activity of trypsin is achieved within the pH range of 7 to 9. The inclusion of phenol red within the specified pH range, gives the solution its pink color.
• Diluent – Concentrated trypsin is solubilized or diluted in a buffered salt solution that contains no Ca2+ or Mg2+, such as Hank’s Balanced Salt Solution, which maintains pH and osmotic balance. Some trypsin products have 0.9% NaCL as diluent instead of Hank’s Balanced Salt Solution.
• Concentration – Based on the cell type and application trypsin is used in various concentrations. For strongly adherent cell lines, trypsin of 2.5 % to 0.25% (10X to 1X power) is used. For studies requiring the integrity of cell surface proteins, lower concentrated (0.05% trypsin) solutions are employed.

Trypsin is a proteolytic enzyme produced by the pancreas to breakdown proteins into amino acids for digestion and other biological processes. The pH and temperature of Trypsin affect its ability to function, pH is determined by the temperature of the solution and formulation. Trypsin in an inactive state has a pH of 3 and in an active state has a pH of 8.