Often used in proteomic research, Trypsin is an enzyme that breaks down proteins to separate or detach cells from the culture vessel. This process is important when cells need to move from vessel to vessel, and when complete, the cells will become suspended and appear rounded.
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|Product Type:||Trypsin Enzyme|
|Color:||Clear, Red Solution|
|pH:||7.0 - 7.4|
|Product Number||Product Name||Spec Sheet||Safety Data Sheet|
|E1510||Trypsin EDTA Solution (10X) in HBSS Solution||Download||Download|
|E1508||Trypsin 0.02% and EDTA 0.05% Solution||Download||Download|
|E1507||Trypsin Solution 2.5% (1X) in HBSS, w/o Calcium & Magnesium||Download||Download|
|E1506||Trypsin - EDTA 0.25% Solution in HBSS, w/o Calcium & Magnesium||Download||N/A|
|E1505||Trypsin - EDTA Soln.(1x) in HBSS||N/A||Download|
|E15032||Trypsin - (0.5 g/L)-EDTA (0.2 g/L) Solution (1X) in Normal Saline||N/A||N/A|
|E15031||Trypsin - EDTA (1x) w/o HBSS||N/A||N/A|
|E1503||Trypsin - EDTA (1x) in HBSS||Download||N/A|
How Do You Handle Trypsin? Is It Safe?
There are no known hazards with Trypsin products, but it is always best to use industrial hygiene measures when in contact with any biological product and to handle product using aseptic techniques.
How Do You Store and Transport Trypsin?
Trypsin products are supplied in gamma irradiated, sterile PETG or PETE bottles and are available in customizable sterile LDPE bags. Biologos recommends that Trypsin products be stored frozen at a temperature range of -50C to -100C.
Trypsin products are shipped frozen by next day air in temperature-controlled LTL freight delivery trucks.
How Do You Prepare Trypsin?
Trypsin is manufactured in an aseptic environment and tested for 9 CFR 113.26 Sterility. Raw Trypsin powder is screened for porcine viruses and mycoplasma per 9 CFR 113.53c.
What Are the Uses for Trypsin?
Trypsin has many effectives uses and is often used in the animal health & Human pharma worlds for microbiological media and cell harvesting. Trypsin is commonly used to develop cell and tissue culture protocols and identify proteins, but can be formulated with inhibitors or activators for use in other applications, such as:
Why Is Trypsin Used in Cell Culture?
Within the human & animal health pharma worlds, Trypsin can be used in research to convert proteins into peptides.
What Are Common Trypsin Enzyme Formulation Options (Additives and Concentrations)?
Biologos offers Trypsin enzyme products in multiple formulations and concentrations (see below), customizable container sizes (100 ml, 500 ml, 1 Liter, 20 Liter, etc.) and varied container types (sterile bottles, sterile bags, etc.). Based on the application and cell types, trypsin is produced with various constituents and at various concentration. The most common variations are:
What is Trypsin?
Trypsin is a proteolytic enzyme produced by the pancreas to breakdown proteins into amino acids for digestion and other biological processes. The pH and temperature of Trypsin affect its ability to function, pH is determined by the temperature of the solution and formulation. Trypsin in an inactive state has a pH of 3 and in an active state has a pH of 8.