The enzyme trypsin has a long and fascinating history dating back to the mid-19th century. Trypsin was first discovered by William Saville Kent, an English biochemist, in 1866 while studying the pancreas.
L-Glutamine is an amino acid found in many foods, and it was first discovered in the late 1800s. It was first isolated in wheat protein in 1927 and its structure was determined in 1935. This was when the term L-glutamine was first used to refer to this amino acid.
Dulbecco’s Modified Eagle Medium (DMEM) is a nutrient medium used to culture cells, developed in the 1960s by Italian-American virologist and Nobel laureate Dr. Giuseppe Bertani. DMEM was designed to be a more versatile version of a previous medium, Eagle's Minimum Essential Medium (MEM), which was first developed by Earl R. Eagle in 1959.
PBS or phosphate-buffered saline is a buffer solution that is particularly valuable because it mimics the ion concentration, osmolarity, and pH of human body fluids. In other words, it's isotonic to human solutions, so it's less likely to cause cell damage, toxicity, or unwanted precipitation in biological, medical, or biochemical research.
